3D Reconstruction of Histological Samples

Specimens were photographed with a Canon 600D Camera mounted on a Zeiss- Stemi 2000. Serial paraffin and semithin sections were analyzed with an Olympus microscope (BX-51) and photographed with an Olympus camera (Olympus cc12) equipped with the dot slide system (2.2 Olympus, Hamburg) and aligned using Imod [71 (link)] and Imod align (http://www.q-terra.de/biowelt/3drekon/guides/imod_first_aid.pdf). 3D- reconstruction of histological sections were performed using Fiji (1.45b) [72 (link)]/ trakem [73 (link)] and Amira (5.0). 3D reconstructions and volume renderings of the μCT scans were performed using Amira 5.0.

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Beckers P., Helm C., Purschke G., Worsaae K., Hutchings P, & Bartolomaeus T. (2019). The central nervous system of Oweniidae (Annelida) and its implications for the structure of the ancestral annelid brain. Frontiers in Zoology, 16, 6.

Publication 2019

600D Camera dot slide system

First aid Imod Microscope Paraffin Reconstructions Scans Stemi

Corresponding Organization :

Other organizations : University of Bonn, University of Göttingen, Osnabrück University, University of Copenhagen, Australian Museum

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3D reconstructions and volume renderings of the μCT scans

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