The urinary pH of the cats was measured at 07.00 hours (fasting pH) and 12.00 hours (postprandial pH). The measurements were performed with an electronic pH meter (Seven Multi, Mettler-Toledo GmbH, Schwerzenbach, Switzerland).
For the analysis of urine composition, the urine was defrosted at room temperature. After measuring the total urine volume of each cat, a sample of 12 ml was taken for further analysis and the remainder frozen at -80°C. The pH of the sample was adjusted to a pH of 2 using hydrochloric acid (37%) (pH-meter Seven Multi, Mettler-Toledo). The added amount of hydrochloric acid was documented. The urine was filtered (Syringe Filter, Bulk, SFCA (surfactant-free cellulose acetate), 0,2μm, 25mm non-sterile, Thermo Scientific, Rochester, NY, USA) prior to the further analysis [23 (link)].
For the analysis of urine composition, the urine was defrosted at room temperature. After measuring the total urine volume of each cat, a sample of 12 ml was taken for further analysis and the remainder frozen at -80°C. The pH of the sample was adjusted to a pH of 2 using hydrochloric acid (37%) (pH-meter Seven Multi, Mettler-Toledo). The added amount of hydrochloric acid was documented. The urine was filtered (Syringe Filter, Bulk, SFCA (surfactant-free cellulose acetate), 0,2μm, 25mm non-sterile, Thermo Scientific, Rochester, NY, USA) prior to the further analysis [23 (link)].
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