The method to culture cardiac myocytes has been described previously[10 (link)]. Transductions with Ad-tf-LC3 and Ad-GFP-LC3 (Hanbio Inc, China) were carried out for 24 hours. Knockdown adenoviruse of Ad-sh-Sirt1 was transduced for 96 hours. Adenoviruses were transduced at 50 MOI [10 (link)].
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