Kidney Function Assessment in Agricultural Workers

In both cohorts, questionnaire data and morning blood samples were collected immediately before and at end of harvest (November and April, respectively). Blood samples were drawn by phlebotomists. Serum was separated, frozen at −77°C and shipped to Lund, Sweden, for analysis. Creatinine and cystatin C were analysed in serum at the Department of Clinical Chemistry of the Skåne University Hospital in Lund, Sweden, using the Cobas 701-instrument (Roche Diagnostics, Basel, Switzerland). Creatinine was measured using an IDMS-calibrated, enzymatic colorimetric method. Cystatin C was measured using a particle enhanced immunoturbidimetric assay, which was standardised against the international reference material ERM-DA471/IFCC. Samples from before and end of harvest from each cohort were analysed in the same session, in order to eliminate analysis batch effects introducing spurious cross-harvest eGFR changes. All analyses were performed in the same laboratory for both the Nicaraguan and Salvadoran samples but at different time points. The laboratory is accredited according to ISO 15189. The details of analysis were also reported previously.6 24 25 (link)

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Andersson A., Hansson E., Ekström U., Grubb A., Abrahamson M., Jakobsson K, & Xu Y. (2022). Large difference but high correlation between creatinine and cystatin C estimated glomerular filtration rate in Mesoamerican sugarcane cutters. Occupational and Environmental Medicine, 79(7), 497-502.

Publication 2022

Cobas 701-

Blood Colorimetric Creatinine Cystatin c Diagnostics Egfr Enzymatic Frozen Immunoturbidimetric assay Serum

Corresponding Organization :

Other organizations : Action Network, University of Gothenburg, Lund University, Sahlgrenska University Hospital

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Variable analysis

independent variables

Harvest period (before and end of harvest)

dependent variables

Creatinine
Cystatin C

control variables

Sample collection and handling (blood samples drawn by phlebotomists, serum separated, frozen at -77°C, and shipped to Lund, Sweden)
Laboratory analysis (conducted at the Department of Clinical Chemistry, Skåne University Hospital, Lund, Sweden, using the Cobas 701-instrument and standardized methods)
Timing of analysis (samples from before and end of harvest for each cohort were analyzed in the same session to eliminate batch effects)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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