SDS-PAGE and Western Blot Analysis of Pfs48/45 Variants

SDS-PAGE and western blot analysis of plant produced Pfs48/45 and Pfs48/45-10C were performed as described previously^{24 (link)}. The FLAG tagged Pfs48/45-10C and Pfs48/45 variants were detected using anti-FLAG mAbs (Cat. No. 637301, BioLegend) or the anti-Pfs48/45 mAb (MRA-316) antibody. Native PAGE of FLAG tagged Pfs48/45 or Pfs48/45-10C variants is performed using native samples and running buffers without denaturants or SDS as described previously^{24 (link)}. The images of the protein bands were taken using the GeneSnap software on a GeneGnome and were quantified using the Gene Tools software (Syngene Bioimaging, UK).

Free full text: Click here

Mamedov T., Cicek K., Miura K., Gulec B., Akinci E., Mammadova G, & Hasanova G. (2019). A Plant-Produced in vivo deglycosylated full-length Pfs48/45 as a Transmission-Blocking Vaccine Candidate against malaria. Scientific Reports, 9, 9868.

Publication 2019

GeneGnome Gene Tools software

Antibody Buffers Gene Native page Plant Protein Sds page Western blot analysis

Corresponding Organization : Akdeniz University

Other organizations : National Institutes of Health, National Institute of Allergy and Infectious Diseases

Top 5 similar protocols

Variable analysis

independent variables

SDS-PAGE and western blot analysis of plant produced Pfs48/45 and Pfs48/45-10C
Native PAGE of FLAG tagged Pfs48/45 or Pfs48/45-10C variants

dependent variables

Detection of FLAG tagged Pfs48/45-10C and Pfs48/45 variants using anti-FLAG mAbs or the anti-Pfs48/45 mAb (MRA-316) antibody
Quantification of protein bands using the Gene Tools software

control variables

Running buffers without denaturants or SDS for native PAGE as described previously

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!