Antibodies against EZH1/2, SUZ12, JARID2, and EED were previously characterized33 (link),63 (link). RBAP48 mouse mAb (GWB-C12FDE) was purchased from GenWay Biotech; H3 mAb (39163) and H3K27me2 mAb (61435) were purchased from Active Motif. Polyclonal rabbit one against H3 from Cell Signaling Technology (9715) ; H3K9me2 (ab1220) and H3K27Ac (ab 4729) were purchased from Abcam; H3K27me1 mouse mAb C0321 from Active Motif; H3K27me3 Rabbit mAb C36B11 (9733), H3K4me3 Rabbit mAb C42D8 (9751), Rabbit mAb D7C6X (14129), Rabbit mAb H2AK119ub (8240 S) and mouse mAb10E2 HDAC1 (5356 S) from Ozyme (Cell Signaling Technology). hEZHIP (HPA006128) and mAb Flag-M2 (F1804) were purchased from SIGMA; Anti-Germ cell-specific Rabbit Polyclonal DPP3A/Stella (19878) and TRA98 (Ab82527) Rat monoclonal one from Abcam. For Immunofluorescence, EED was detected with the M26 antibody. Antibody against mEZHIP was raised against the two following synthetic peptides: CAESSRAESDQSSPAG (corresponding to a.a. 91–106) and CAQSAGRNLRPRPRSS (corresponding to a.a. 192–206). Anti-mouse β-TUBULIN was purchased from Invitrogen 32–2600.
Primary antibodies were diluted 1:3000 for WB analysis and 1:250 for Immunostaining.
Primary antibodies were diluted 1:3000 for WB analysis and 1:250 for Immunostaining.
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