Histological Analysis of Mouse Organs

Organs (liver, spleen, and kidney) were harvested from mice and fixed in neutral buffered formalin (10%) and subsequently embedded in paraffin to generate tissue blocks. These blocks were then sectioned at a thickness of 4 μm and stained using Harris haematoxylin (Leica Surgipath) and alcoholic eosin-Y stain (Leica Surgipath). Staining was executed using the automated Leica autostainer-XL platform. Upon staining, the sections were subsequently analyzed by two pathologists on a brightfield microscope to determine morphology and architectural coherence. These pathological assessments were performed in a blinded manner. Detailed and comprehensive analysis of cellular and -subcellular level changes of organs were performed as previously described (16 (link)).