Phos-tag gels were prepared and run as previously described (Gavagan et al., 2020 (link)). After electrophoresis, the gel was incubated 3 x with transfer buffer +10 mM EDTA for 10 min before the transfer to increase transfer efficiency. Protein levels were detected with anti-MBP antibody (Cell Signaling Technology #2396; Figure 2—figure supplement 9A ). The secondary antibody was IRDye 800CW Donkey Anti-Mouse IgG antibody (Li-Cor #926–32212).
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