Plasma Whole-Genome Library Preparation
Corresponding Organization :
Other organizations : Memorial Sloan Kettering Cancer Center
Variable analysis
- Amount of cell-free DNA (cfDNA) used for library preparation (5–250 ng)
- Whole-genome sequencing of the prepared libraries
- Cell-free DNA extraction using the QIAamp Circulating Nucleic Acid Kit
- Library preparation using the NEBNext DNA Library Prep Kit
- Sequencing using 100 bp paired-end runs on HiSeq 2000/2500 sequencers (Illumina)
- No positive or negative controls were explicitly mentioned in the provided information.
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