In a microwave vial, tert-butyl {2-[5-methyl-2-(pyridin-3-yl)-1,3-thiazol-4-yl]-2-oxoethyl}carbamate
(4.29 g, 12.87 mmol) was dissolved in 30 mL of methanol. Potassium
cyanide (3.35 g, 51.47 mmol) and ammonium carbonate (4.95 g, 51.47
mmol) were added. The vial was sealed, and the mixture was stirred
at 80 °C overnight. The salts were filtered off and rinsed with
methanol. The filtrate was concentrated. The crude product was purified
by preparative HPLC. Product containing samples were united, the solvents
were evaporated with a rotary evaporator and the residue was lyophilized.
1.97 g (100% purity, 38% yield) of the title compound was obtained.
LC–MS (Method 2): Rt = 1.21 min; MS (ESIpos): m/z = 404 [M + H]+.
(4.29 g, 12.87 mmol) was dissolved in 30 mL of methanol. Potassium
cyanide (3.35 g, 51.47 mmol) and ammonium carbonate (4.95 g, 51.47
mmol) were added. The vial was sealed, and the mixture was stirred
at 80 °C overnight. The salts were filtered off and rinsed with
methanol. The filtrate was concentrated. The crude product was purified
by preparative HPLC. Product containing samples were united, the solvents
were evaporated with a rotary evaporator and the residue was lyophilized.
1.97 g (100% purity, 38% yield) of the title compound was obtained.
LC–MS (Method 2): Rt = 1.21 min; MS (ESIpos): m/z = 404 [M + H]+.
