Ferroptosis Induction and Measurement

The following compounds were used: polybrene, puromycin, buthionine sulfoxamine, paraquat, L-ascorbic acid (Sigma-Aldrich), blasticidin (Invivogen), RSL3 (Selleckchem), ML162 (Aobious), erastin (Tocris), ML210^{51 (link)} (Selleckchem), dithiothreitol (Biovision), ferrostatin-1 (Tocris), and matrigel (Corning), Egg PC (Sigma), STY-BODIPY^{35 (link)}, DTUN^{32 (link)}, α-tocopherol (Sigma, purified by flash chromatography before use), CoenzymeQ10 (Alfa Aesar), tetrahydrobiopterin (BH4) and dihydrobiopterin (BH2) (Cayman Chemicals), NADPH (Alfa Aesar), dihydrofolate reductase (R&D Systems), methotrexate (US Biological), superoxide dismutase (Sigma), catalase (Sigma). All purchased compounds had a reported purity value ≥ 98%. QM385 was kindly provided by Dr. Clifford Woolf.

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Soula M., Weber R.A., Zilka O., Alwaseem H., La K., Yen F., Molina H., Garcia-Bermudez J., Pratt D.A, & Birsoy K. (2020). Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers. Nature chemical biology, 16(12), 1351-1360.

Publication 2020

L-ascorbic acid blasticidin ML162 erastin matrigel Egg PC α-tocopherol superoxide dismutase catalase

Biological Buthionine Catalase Cayman Chromatography Dihydrobiopterin Dihydrofolate reductase Dithiothreitol Erastin Ferrostatin 1 L ascorbic acid Matrigel Methotrexate Nadph Paraquat Polybrene Puromycin Superoxide dismutase Tetrahydrobiopterin α tocopherol

Corresponding Organization :

Other organizations : Rockefeller University, University of Ottawa

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Variable analysis

independent variables

Polybrene
Puromycin
Buthionine sulfoxamine
Paraquat
L-ascorbic acid (Sigma-Aldrich)
Blasticidin (Invivogen)
RSL3 (Selleckchem)
ML162 (Aobious)
Erastin (Tocris)
ML210 (Selleckchem)
Dithiothreitol (Biovision)
Ferrostatin-1 (Tocris)
Matrigel (Corning)
Egg PC (Sigma)
STY-BODIPY
α-tocopherol (Sigma, purified by flash chromatography before use)
Coenzyme Q10 (Alfa Aesar)
Tetrahydrobiopterin (BH4)
Dihydrobiopterin (BH2) (Cayman Chemicals)
NADPH (Alfa Aesar)
Dihydrofolate reductase (R&D Systems)
Methotrexate (US Biological)
Superoxide dismutase (Sigma)
Catalase (Sigma)
QM385 (provided by Dr. Clifford Woolf)

dependent variables

Not explicitly mentioned

control variables

All purchased compounds had a reported purity value ≥ 98%

positive controls

Not specified

negative controls

Not specified

Annotations

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