Microtiter-plate technique [11 (link)] with the crystal violet (CV) assay was used [10 (link)]. For determination of EOPH action in the biofilm, the plates were subjected to reading the optical density using a microplate reader (Molecular Devices-Spectra Max Paradigm Multi-Mode) at a wavelength of 595 nm. The activity was found in concentrations that did not observe the adhesion of crystal violet in any of the wells.
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