Flower extracts were prepared as described by Grzeszczuk et al. [46 (link)] with modifications. The dried plant material was ground in a grinder, and then the homogenized material (0.5 g) was supplemented with 40 mL of 80% methanol and deionized water in 7:3 ratio (v/v). The samples were placed in an ultrasonic bath for 30 min. The extracts were centrifuged for 5 min at 5000× g (Centrifuge 5418 Eppendorf, Warsaw, Poland) and filtered through 0.22 µm nylon membrane filters (Merck, Darmstadt, Germany). All the extractions were prepared in triplicate. The final extracts were stored at −20 °C.
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