First, a 4% sodium alginate solution was prepared from distilled water and alginic acid sodium salt. It was used throughout the experiment for emulsion preparation as the shell material. Emulsion with Trifolium pratense L. and Glycyrrhiza glabra L. extracts, and Myristica fragrans Houtt. essential oil were prepared as follows: solution with excipients (maltodextrin, inulin, and/ or gum Arabic) was mixed with sodium alginate solution (stirred for 15 min with a magnetic stirrer MSH-20A (Witeg, Wertheim, Germany)) and then extracts with essential oil were added. The solution was homogenised for 15 min at 5000 rpm using an IKA T18 homogeniser (IKA-Werke GmbH & Co., KG, Staufen, Germany).
The emulsion’s stability was tested using a centrifuge Sigma 3-18KS (Sigma Laborzentrifugen GmbH, Osterode am Harz, Germany). The test was repeated three times using 23 °C temperature, 3000 rpm, and the duration was 5 min. The centrifugation index (CI) was calculated to evaluate emulsion stability.
where Ve is the volume of the remaining emulsion after centrifugation and Vi is the volume of the initial emulsion.
The emulsion’s stability was tested using a centrifuge Sigma 3-18KS (Sigma Laborzentrifugen GmbH, Osterode am Harz, Germany). The test was repeated three times using 23 °C temperature, 3000 rpm, and the duration was 5 min. The centrifugation index (CI) was calculated to evaluate emulsion stability.
where Ve is the volume of the remaining emulsion after centrifugation and Vi is the volume of the initial emulsion.
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