Acetylshikonin and 3-DSC Cytotoxicity Assay

Cells (5 × 10³ cells/well) were seeded in 96-well plates and treated with different concentrations (5 µM, 10 µM, 20 µM, 40 µM, 80 µM) of acetylshikonin or 3-DSC. Cell proliferation was detected at various times (24, 48, and 72 h). For each well, 20 µl of the MTS solution (Promega, Madison, WI) was added. After 1 h of incubation, 25 µl of 10% SDS solution was added, and the absorbance was detected at 492 and 690 nm with a microplate spectrophotometer [22 (link)].

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Cui J., Guo R., Wang Y., Song Y., Song X., Li H., Song X, & Li J. (2022). Acetylshikonin suppresses diffuse large B-Cell Lymphoma cell growth by targeting the T-lymphokine-activated killer cell-originated protein kinase signalling pathway. Bioengineered, 13(2), 4428-4440.

Publication 2022

MTS solution

Acetylshikonin Cell proliferation Cells Promega

Corresponding Organization : First Affiliated Hospital of Zhengzhou University

Other organizations : Zhengzhou University

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Variable analysis

independent variables

Concentration of acetylshikonin or 3-DSC (5 µM, 10 µM, 20 µM, 40 µM, 80 µM)

dependent variables

Cell proliferation

control variables

Cell seeding density (5 × 10^3 cells/well)
Incubation time (24, 48, and 72 h)
MTS solution addition (20 µl)
SDS solution addition (25 µl)
Absorbance detection (492 and 690 nm)

controls

No additional controls were explicitly mentioned in the input protocol.

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