Characterization of Patient-Derived Glioblastoma Cell Lines

Twelve patient-derived glioblastoma cell lines were kindly provided by the QIMR Berghofer Medical Research Institute (Brisbane, Australia). The cell lines are fully characterised with publicly available molecular and patient data, published by Stringer et al. [13 (link)]. Cells were grown as adherent monolayers in Matrigel^® (Corning^®, Corning, NY, USA)-coated tissue culture flasks in StemPro^® NSC SFM (Gibco^TM, Waltham, MA, USA) containing 100 I.U./mL penicillin and 100 µg/mL streptomycin (Gibco^TM, Waltham, MA, USA), and incubated at 37 °C in 5% CO₂/95% humified air. Cells were passaged using StemPro^® Accutase^® solution (Gibco^TM, Waltham, MA, USA) for detachment of adherent cells. TMZ was purchased from Sigma-Aldrich (Burlington, MA, USA), aliquoted in dimethyl sulfoxide (DMSO) (100 mM) and stored between 2 and 8 °C. RT was delivered using a medical linear accelerator (LINAC) at GenesisCare, Gateshead NSW (Australia) or RS-2000 Small Animal Irradiator (Rad Source, Buford, GA, USA).