Zebrafish embryos were exposed to eriocitrin from 6 hpf to 5 dpf. The number of survivors was counted under a MZ16F stereoscopic microscope (Leica Microsystems). One hour before evaluation of locomotor activity, a fish was removed from each experimental unit and placed in a well with 50 μl of E3 medium in a 96-well, clear-bottom plate for acclimatization. The fish were then video-recorded for 15 min. The resulting footage was evaluated to measure the swimming distance using the EthoVision XT system ver8.0 (Noldus Information Technology, Wageningen, The Netherlands) as described previously60 (link).
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Zebrafish Embryo Exposure to Eriocitrin
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Corresponding Organization :
Other organizations : Mie University, Sumitomo Dainippon Pharma (Japan)
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Variable analysis
independent variables
- Eriocitrin exposure
- Number of survivors
- Swimming distance
- E3 medium
- 96-well, clear-bottom plate
- Acclimatization period of 1 hour before video recording
- Video recording duration of 15 minutes
- No positive or negative controls were explicitly mentioned in the input protocol.
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