Investigating the Role of PI3K in FGF23-Mediated Neuronal Oxidative Stress

oeNC- or oeFGF23-infected cells were seeded into 6-well plates (5x10⁵ cells/well) and divided into the following groups: OeNC, cells infected with oeNC; oeFGF23, cells infected with oeFGF23; LY294002, cells infected with oeNC and incubated with 10 µM LY294002 (PI3K inhibitor; MedChemExpress) at 37˚C for 12 h (19 (link)); and oeFGF23 + LY294002, cells infected with oeFGF23 and incubated with 10 µM LY294002 at 37˚C for 12 h. Primary neurons without infection or inhibitor treatment were used as the control group. The primary neurons in all groups were then stimulated with 100 µM H₂O₂ for 24 h at 37˚C. Finally, the cells were harvested for RT-qPCR, western blotting and apoptosis analysis.