Immunoblot and dot-blot analysis of mice and human brain tissues were performed as previously described (Lee et al., 2002 (link); Li et al., 2004 (link); Li et al., 2005 (link); Martin et al., 2006 (link); Wang et al., 2008 (link)). For dot-blot analysis, lysates were spotted directly on the nitrocellulose membrane and let it dry completely. Immunoreactivity was visualized using chemiluminescence detection (Pierce, Rockford, IL) after incubations with the appropriate horseradish peroxidase-conjugated secondary antibody, using a CCD based Biorad Molecular Imager ChemiDoc XRS+ System (Biorad, Hercules, CA) or X-ray films. The immunoreactive band intensities were determined by densitometry or the Quantity One software (Biorad, Hercules, CA).
Following antibodies were used: grp78, grp94, (Stressgen, Ann Arbor, MI); Syn-1, cytochrome C (BD Transduction Laboratories, Franklin Lakes, NJ); A11 (Kayed et al., 2003 (link)); pser129-αS (Fujiwara et al. 2002 (link)); syn303 (Duda et al., 2000 (link)); FILA-1 (Lindersson et al., 2004 (link)).
Following antibodies were used: grp78, grp94, (Stressgen, Ann Arbor, MI); Syn-1, cytochrome C (BD Transduction Laboratories, Franklin Lakes, NJ); A11 (Kayed et al., 2003 (link)); pser129-αS (Fujiwara et al. 2002 (link)); syn303 (Duda et al., 2000 (link)); FILA-1 (Lindersson et al., 2004 (link)).
