Analysis of gait and posture was performed with the CatWalk XT automated system as mentioned in our previous publications (Noldus) (42 (link), 70 (link)). Acquisition of data took place in a darkened room with red light. The CatWalk apparatus records print position, gait postures, and weight distribution through its green illuminated walkway. A minimum of three valid runs, complete crossings with no turns or scaling of sidewalls, were obtained for each tested mouse. Runs that did not comply to the preset standards were excluded from the final analysis. The regularity index (%) expresses the number of normal step sequence patterns relative to the total number of paw placements. The regularity index is a fractional measure of interpaw coordination. In healthy, fully coordinated animals, its value is 100%. Stand (in seconds) is the duration of contact with the glass plate of the print. Stride length (in centimeters) is the distance (in distance units) between successive placements of the same paw. Swing speed (in centimeters per second) is the speed (in distance units per second) of the paw during swing (swing speed = stride length / swing). The body speed (in centimeters per second) of a step cycle of a specific paw is calculated by dividing the distance that the animal’s body traveled from one initial contact of that paw to the next by the time to travel that distance. Step cycle (in seconds) is the time between two consecutive initial contacts of the same paw (step cycle = stand + swing).