Cytochrome c (C7752) from equine heart (≥99%),
deuterium oxide (D2O, 99.99%), ammonium acetate (≥98%),
and formic acid (≥98%) were purchased from Sigma-Aldrich (St.
Louis, MO). Tau protein was expressed from Escherichia
coli BL21 cells containing a pET-29b vector encoding
the htau40 isoform. Purification was carried out as previously reported20 (link) and protein was stored in 20% glycerol at −80
°C. Both cytochrome c and tau were resuspended or buffer exchanged
into 50 mM ammonium acetate prior to MS analysis.
deuterium oxide (D2O, 99.99%), ammonium acetate (≥98%),
and formic acid (≥98%) were purchased from Sigma-Aldrich (St.
Louis, MO). Tau protein was expressed from Escherichia
coli BL21 cells containing a pET-29b vector encoding
the htau40 isoform. Purification was carried out as previously reported20 (link) and protein was stored in 20% glycerol at −80
°C. Both cytochrome c and tau were resuspended or buffer exchanged
into 50 mM ammonium acetate prior to MS analysis.
