Mechlorethamine hydrochloride (NM) was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Methylene blue (MB) was purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA). Lanolin (C10‐30 cholesterol/lanosterol esters) and polyethylene glycol 400 were gifted from Croda Inc. (Edison, NJ, USA). NDH‐4338, a cyclooxygenase, inducible nitric oxide synthase, and acetylcholinesterase inhibitor prodrug, was synthesized as described.28 Ultrapure water (Milli‐Q® Advantage A10®, Type 1 water, MilliporeSigma, Burlington, MA, USA, water) was used throughout the study.
Protocol detail
Synthesis and Characterization of NDH-4338
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Mechlorethamine hydrochloride (NM)
Methylene blue
Lanolin (C10‐30 cholesterol/lanosterol esters)
polyethylene glycol 400
Acetylcholinesterase inhibitor
Cholesterol esters
Croda
Cyclooxygenase
Inducible nitric oxide synthase
Lanolin
Lanosterol
Mechlorethamine hydrochloride
Methylene blue
Polyethylene glycol 400
Prodrug
Corresponding Organization :
Other organizations : Rutgers, The State University of New Jersey
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Variable analysis
independent variables
- Mechlorethamine hydrochloride (NM)
- Methylene blue (MB)
- Lanolin (C10‐30 cholesterol/lanosterol esters)
- Polyethylene glycol 400
- NDH‐4338, a cyclooxygenase, inducible nitric oxide synthase, and acetylcholinesterase inhibitor prodrug
- Unspecified
- Ultrapure water (Milli‐Q® Advantage A10®, Type 1 water, MilliporeSigma, Burlington, MA, USA, water)
- Positive control: Unspecified
- Negative control: Unspecified
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