Antibodies against phospho-AMPK (2531) at Thr172, AMPK (2603), phospho-IKKα (Ser176)/IKKβ (Ser177; 2078S), IKKα (2682), IKKβ (2370), phospho-IκB at Ser32/36 (9246), IκB (4814), phospho-ULK1 at Ser317 (6887), and ULK1 (8054) were from Cell Signaling. Anti-PGC1α (ST1204) was purchased from Millipore. Antibodies against AdipoR1 (ab126611), AdipoR2 (ab77612), UCP1 (ab23841), and C/EBPβ (ab32358) were from Abcam. Anti-LC3B (L7543) from Sigma was used for Western blot, analysis and Anti-LC3 pAb (PM036) from MBL International was used for staining. Anti-adiponectin and anti-β-tubulin were kindly provided by Dr. Lily Dong (University of Texas Health Science Center at San Antonio, San Antonio, TX) as described previously (Alsted et al., 2009 (link); Meng et al., 2017 (link)). AdipoRon (924416–43-3) and 5Z (253863–19-3) were obtained from Cayman Chemical Company. IRAK 1/4 inhibitor I (I5409) and rapamycin (R8781) were from Sigma-Aldrich. 3-MA (189490) was purchased from EMD Millipore. Two recombinant mouse adiponectin (full-length and mutated C39A) proteins (ab62957 and ab94676) were purchased from Abcam. Mouse ST2/IL-1 R4 antibody (MAB10041) was from R&D Systems.
Protocol detail
Adiponectin Signaling and Autophagy Regulation
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Adiponectin
Adiporon
Antibodies
Antibody
Cayman
Ikkα
Ikkβ
Irak 1
Lily
Mouse
Mouse adiponectin
Pgc1α
Proteins
Rapamycin
Tubulin
Ucp1
Ulk1
Western blot
Corresponding Organization : University of New Mexico
Other organizations : Central South University, Second Xiangya Hospital of Central South University, The University of Texas at San Antonio, The University of Texas Health Science Center at San Antonio
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Variable analysis
independent variables
- AdipoRon (924416–43-3)
- 5Z (253863–19-3)
- IRAK 1/4 inhibitor I (I5409)
- Rapamycin (R8781)
- 3-MA (189490)
- Phospho-AMPK (2531) at Thr^172
- AMPK (2603)
- Phospho-IKKα (Ser^176)/IKKβ (Ser^177; 2078S)
- IKKα (2682)
- IKKβ (2370)
- Phospho-IκB at Ser^32/36 (9246)
- IκB (4814)
- Phospho-ULK1 at Ser^317 (6887)
- ULK1 (8054)
- PGC1α (ST1204)
- AdipoR1 (ab126611)
- AdipoR2 (ab77612)
- UCP1 (ab23841)
- C/EBPβ (ab32358)
- LC3B (L7543)
- LC3 (PM036)
- Adiponectin
- Two recombinant mouse adiponectin (full-length and mutated C39A) proteins (ab62957 and ab94676)
- Mouse ST2/IL-1 R4 antibody (MAB10041)
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