Native ChIP was performed as previously described using 5×106 MDA-MB-231 cells (40 (link)). A spike-in strategy was used to normalize all ChIP-seq data to reduce the effects of technical variation and sample processing bias. Spike-In chromatin (Active Motif, 53083) and Spike-in antibody (Active Motif, 61686) were used according to manufacturer instructions.
Protocol detail
Native ChIP-seq with Spike-in
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Corresponding Organization : Union Hospital
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Variable analysis
independent variables
- Cell line: MDA-MB-231 cells
- ChIP-seq data
- Spike-in chromatin (Active Motif, 53083)
- Spike-in antibody (Active Motif, 61686)
- Positive control: Spike-in chromatin and spike-in antibody used to normalize ChIP-seq data and reduce technical variation and sample processing bias
- Negative control: Not explicitly mentioned
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