Immunoblotting of left ventricular lysates was performed as previously described.25 (link) Anti-SERCA (1:5000, A010–20, Badrilla), RyR2 (1:2500, ARR002, Alomone), PLB (1:5000, A010–14, Badrilla), PLB-Thr17 (1:5000, A010–13, Badrilla), PLB-Ser16 (1:5000, A010–12, Badrilla), and α-tubulin (1:1000, ab52866, Abcam) were used as primary antibodies. Anti-rabbit or anti-mouse 680nm or 800nm were used as secondary antibodies (IRDyes 680LT or 800CW, Licor Biosciences).
Protocol detail
Immunoblotting of Left Ventricular Lysates
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Corresponding Organization :
Other organizations : University of California, Davis, University of Kentucky
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Variable analysis
independent variables
- Anti-SERCA (1:5000, A010–20, Badrilla)
- RyR2 (1:2500, ARR002, Alomone)
- PLB (1:5000, A010–14, Badrilla)
- PLB-Thr17 (1:5000, A010–13, Badrilla)
- PLB-Ser16 (1:5000, A010–12, Badrilla)
- α-tubulin (1:1000, ab52866, Abcam)
- Immunoblotting of left ventricular lysates
- Anti-rabbit or anti-mouse 680nm or 800nm secondary antibodies (IRDyes 680LT or 800CW, Licor Biosciences)
- Positive controls not explicitly mentioned.
- Negative controls not explicitly mentioned.
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