First, US was used to identify muscle alignment and locate the rectus femoris, vastus lateralis, and vastus medialis. Next, we initiated the measurement function for SWE and set the region of interest (ROI) to 3 mm. We selected five points on each image, including the midpoint and the four corners to measure SWEmean. We took the mean of three replicate measurements for further analysis (
Measurement of US parameters and SWEmean.