Immortalized Hepatocyte Culture Protocol

Immortalized embryonic hepatocytes were cultured in Medium 199 (WelGENE) that was supplemented with 10% fetal bovine serum (WelGENE) and 1% penicillin-streptomycin (WelGENE), as previously described (Back et al., 2009 (link)). AML12 muse normal hepatocytes were obtained from the American Type Culture Collection (ATCC, USA). The cells were cultured in DMEM/F12 (WelGENE) that was supplemented with 10% fetal bovine serum (WelGENE), 100 nM dexamethason (Sigma-Aldrich), 1% insulin-transferrin-selenium-pyruvate supplement (ITSP; WelGENE), and 1% penicillin-streptomycin (WelGENE). The Lenti-X 293T Cell Line (Clontech, USA) was cultured in DMEM (WelGENE) containing 10% fetal bovine serum (WelGENE) and 1% penicillin-streptomycin (WelGENE).

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Kim M.J., Choi W.G., Ahn K.J., Chae I.G., Yu R, & Back S.H. (2020). Reduced EGFR Level in eIF2α Phosphorylation-Deficient Hepatocytes Is Responsible for Susceptibility to Oxidative Stress. Molecules and Cells, 43(3), 264-275.

Publication 2020

Medium 199 fetal bovine serum penicillin-streptomycin DMEM/F12 dexamethason insulin-transferrin-selenium-pyruvate supplement (ITSP; Lenti-X 293T Cell Line DMEM

Cell line Cells Embryonic Fetal bovine serum Hepatocytes Insulin Muse Penicillin Pyruvate Selenium Streptomycin Supplement Transferrin

Corresponding Organization :

Other organizations : University of Ulsan

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Variable analysis

independent variables

Cell type (immortalized embryonic hepatocytes, AML12 muse normal hepatocytes, Lenti-X 293T Cell Line)

dependent variables

Not explicitly mentioned

control variables

Medium 199 (WelGENE) supplemented with 10% fetal bovine serum (WelGENE) and 1% penicillin-streptomycin (WelGENE) for immortalized embryonic hepatocytes
DMEM/F12 (WelGENE) supplemented with 10% fetal bovine serum (WelGENE), 100 nM dexamethason (Sigma-Aldrich), 1% insulin-transferrin-selenium-pyruvate supplement (ITSP; WelGENE), and 1% penicillin-streptomycin (WelGENE) for AML12 muse normal hepatocytes
DMEM (WelGENE) containing 10% fetal bovine serum (WelGENE) and 1% penicillin-streptomycin (WelGENE) for Lenti-X 293T Cell Line

controls

No positive or negative controls were explicitly mentioned in the provided information.

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