CXCR4 Expression Analysis by Flow Cytometry

CXCR4 expression was analyzed by flow cytometry as previously described [23 (link)]. Breifly, the CXCR4 protein was labeled by mouse anti-CXCR4 primary antibody (Santa Cruz Biotech, TX) in 1:20 dilution to a 200 μL final volume of cells (1 × 10⁵ cells/mL). Antibodies used for flow cytometry included anti-mouse-FITC (BD Biosciences, San Jose, CA), and the samples were latter analyzed using a FACS Calibur/LSRII flow cytometer (Becton-Dickinson, Franklin Lakes, NJ). Data were analyzed using BD FACSDiva software. Gates were set according to isotype controls.

Free full text: Click here

Amara S., Majors C., Roy B., Hill S., Rose K.L., Myles E.L, & Tiriveedhi V. (2017). Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS ONE, 12(6), e0180097.

Publication 2017

mouse anti-CXCR4 primary antibody anti-mouse-FITC FACS Calibur/LSRII

Anti antibody Antibodies Cells Cxcr4 Dilution Fitc Flow cytometry Isotype Mouse Protein

Corresponding Organization : Vanderbilt University

Top 5 similar protocols

Variable analysis

independent variables

CXCR4 protein expression

dependent variables

CXCR4 protein expression measured by flow cytometry

control variables

Mouse anti-CXCR4 primary antibody dilution (1:20)
Final volume of cells (200 μL)
Cell density (1 × 10^5 cells/mL)
Anti-mouse-FITC secondary antibody
FACS Calibur/LSRII flow cytometer
BD FACSDiva software for data analysis
Isotype controls for gating

controls

Isotype controls for gating

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!