Transcriptional Profiling of ELO and KCS Genes

To examine the expressions of ELO and KCS genes, tissue-specific RNA-seq data under normal and stressed conditions were retrieved from the European Nucleotide Archive database (https://www.ebi.ac.uk/ena/browser/home). Expression data of seed coat development stages (heart, globular, zygote, octant, bent, torpedo, and mature seed coat) and embryo development stages (heart, globular, zygote, octant, bent, torpedo, unfertilized stage ovule, and mature embryo) under normal conditions were acquired from Bio project PRJNA 641,876. Expression data of Bio project PRJNA 524,852 were used to examine gene expression during seed and embryo development under two different temperatures and day intervals. Root tissue data from Bio project PRJNA 524,852 under zinc and phosphate applications was also explored. Moreover, RNA seq data from Bio project PRJNA 421,190 was used to examine expression patterns under biotic stress of X. campestris.
The expressions of KCS and ELO genes in different tissues were quantified using galaxy Europe server (https://usegalaxy.eu/) and transcripts were evaluated in TPM (Transcripts per kilobase million)^{69 (link)}. Moreover, heat maps were constructed using Morpheus software (https://software.broadinstitute.org/morpheus/).