A segment of the median nerve was obtained from the proximal upper arm of five fresh cadavers, less than 24 h postmortem. The cadavers were donated for research and educational purposes to the Institute of Anatomy, Faculty of Medicine, University of Ljubljana, through a willed cadaver donation program. Each nerve was cut into a 9 mm long segment, had carefully removed the surrounding connective tissue, and inserted in a 10-mm-diameter glass tube. To prevent sample dehydration, the glass tube was filled with perfluorinated liquid, Galden SV90 from Solvay (Brussels, Belgium), which does not produce any detectable MRI signal (Awais et al., 2022 (link)). The study was approved by the National Medical Ethics Committee of the Republic of Slovenia (Permit No: 0120–239/2020/3).
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