Cells were grown on 10 cm dishes and incubated with the compounds for 24 h in full media. The protocol for extraction of Mito-Met analogs was the same as previously described, but without BHT (16 (link)). LC-MS/MS analyses were performed using a Kinetex Phenyl-Hexyl column (50 mm × 2.1 mm, 1.7 μm, Phenomenex) equilibrated with water:acetonitrile mixture (4:1) containing 0.1% formic acid. Compounds were eluted by increasing the content of acetonitrile from 20% to 100% over 4 min and detected using the MRM mode.
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Extraction and LC-MS/MS Analysis of Mito-Met Analogs
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Corresponding Organization : Medical College of Wisconsin
Other organizations : Aix-Marseille Université, Centre National de la Recherche Scientifique, Fundación Cardiovascular de Colombia
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Variable analysis
independent variables
- Compounds
- Extraction of Mito-Met analogs
- LC-MS/MS analyses
- Cells were grown on 10 cm dishes
- Incubation time (24 h)
- Full media
- Kinetex Phenyl-Hexyl column (50 mm × 2.1 mm, 1.7 μm, Phenomenex)
- Water:acetonitrile mixture (4:1) containing 0.1% formic acid
- Acetonitrile content increase from 20% to 100% over 4 min
- MRM mode for detection
- Without BHT
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