Transgenic Mouse Models for PD-1 Signaling

WT C57BL/6J (B6; stock no: 000664), B6(C)-H2-Ab1^bm12/KhEgJ (bm12; stock no: 001162), BALB/cJ (stock no: 000651), and C3H/HeJ (stock no: 000659) mice were purchased from The Jackson Laboratory. Foxp3-GFP mice were a gift from the Alexander Y. Rudensky laboratory (Memorial Sloan Kettering Cancer Center) (50 (link)). Descriptions of PD-1–KO (51 (link)), PD-L1–KO (29 (link)), and T cell–specific PD-1 Tg mice (12 (link)) have been published previously. Briefly, in the PD-1 Tg mice, PD-1 is overexpressed under the hCD2 promotor (12 (link)). PD-1 Tg mice were crossed with Foxp3-GFP animals in our animal facility. All mice were at 8–12 weeks of age and were harbored and used following the Harvard Medical School and National Institutes of Health guidelines.