TARBP2 Plasmid Construction and Knockdown

The pLPC-TARBP2 plasmid was kindly provided by Dr Sonia A. Melo and then subcloned into the vectors pCMV-Tag2b and pCMV-myc. The Flag-TARBP2 was cloned into the lentiviral vector (System Biosciences, Mountain View, CA, USA) carrying EGFP and Puromycin genes28 (link)46 (link). The TARBP2 cDNA was cloned into the prokaryotic expression vector pGEX4T1. The shRNA sequence targeting human TARBP2 3′-UTR (TARBP2sh) was obtained from Sigma ‘Mission shRNA' online: 5′-TCATGGATGTGCACCCTTTG-3′ at 1,604 site. The shRNA was cloned into pLKO.1 vector or pGreenPuro shRNA (System Biosciences). The oligo sequences of other shRNAs including Senp1sh1, Senp1sh2 and SUMO1sh are listed in Supplementary Table 2.