Genetic Screening of IA Susceptibility

To further glean the biological role of S100B in the occurrence of IA, we screened an existing DNA archive for the presence of 3 previously described SNPs (rs9722 [S100B], rs2070600 [AGER], rs1800624 [AGER]) (Cunha et al., 2011 (link)). The archive contains previously collected DNA samples from allogeneic stem cell transplant recipients [33 patients with proven or probable IA and 38 controls without IA, classification according to the EORTC/MSG criteria (De Pauw et al., 2008 (link))]. This archive allowed extending genotyping to a larger number of patients with similar risk for IA, in addition to the relatively limited number of original study patients.
Human genomic DNA was extracted by using the QIAmp Blood DNA Mini Kit (Qiagen), followed by melting curve analyses using a LightCycler®1.5 instrument (Löffler et al., 2000 (link)) and specific hybridization probes (LightSNiP, TIB MOLBIOL).

Free full text: Click here

Dix A., Czakai K., Springer J., Fliesser M., Bonin M., Guthke R., Schmitt A.L., Einsele H., Linde J, & Löffler J. (2016). Genome-Wide Expression Profiling Reveals S100B as Biomarker for Invasive Aspergillosis. Frontiers in Microbiology, 7, 320.

Publication 2016

QIAmp Blood DNA Mini Kit LightSNiP

Ager Allogeneic cell Biological Blood Human genomic Hybridization Patients Snps Stem Transplant recipients

Corresponding Organization : Universitätsklinikum Würzburg

Top 5 similar protocols

Variable analysis

independent variables

S100B SNP (rs9722)
AGER SNP (rs2070600)
AGER SNP (rs1800624)

dependent variables

Occurrence of invasive aspergillosis (IA)

control variables

DNA samples from allogeneic stem cell transplant recipients
EORTC/MSG criteria for classification of IA (proven or probable IA vs no IA)
Extraction of human genomic DNA using QIAmp Blood DNA Mini Kit
Melting curve analyses using LightCycler®1.5 instrument
Specific hybridization probes (LightSNiP, TIB MOLBIOL)

controls

Positive control: DNA samples from patients with proven or probable IA
Negative control: DNA samples from patients without IA

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!