Specimens and clinical data were obtained according to the declaration of Helsinki and the ethics guidelines of the University of Washington Human Subjects Division. All experimental protocols were approved by the University of Washington Human Subjects Division, and informed consent was obtained from all participants. CF patients were recruited from outpatient clinics, but were otherwise not selected with respect to any clinical characteristics or inclusion criteria and consequently reflect a convenience sample (Table 1 ). A convenience sample of healthy volunteers without acute disease were recruited from the general population (Supplemental Table 2 ).
Whole blood was collected from subjects in Cell-Free DNA Blood Collection Tubes (Streck) and processed within 3 hours. Patient sputum samples collected at the time of clinic visits were subjected to microbiological culture by the University of Washington Clinical Microbiology Laboratory according to standard clinical procedures, which are described in detail elsewhere29 (link).
Whole blood was collected from subjects in Cell-Free DNA Blood Collection Tubes (Streck) and processed within 3 hours. Patient sputum samples collected at the time of clinic visits were subjected to microbiological culture by the University of Washington Clinical Microbiology Laboratory according to standard clinical procedures, which are described in detail elsewhere29 (link).
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