Plasmid Constructs for HCV Protein Expression

pHA-PIAS2 was constructed by cloning human PIAS2 (GenBank accession number NM_004671.3) cDNA into pXJ40-HA. The plasmids expressing the FLAG-tagged HCV core, E1, E2, NS2, NS3, NS4A, NS4B, NS5A and NS5B proteins of genotype 2a (JFH1; GenBank accession no AB047639), pNL4.3.lucR⁻E⁻, pcDNA3.1-E1E2 and pHCV-internal ribosome entry site (IRES) were described previously [29 (link),31 (link)]. pUb was constructed by cloning human ubiquitin cDNA into pcDNA3.1. The following plasmids were kindly provided by different groups: human SUMO1, SUMO2/3 expression plasmids from Bing Sun (Institute Pasteur of Shanghai) [19 (link)], pSENP1 (SUMO1/sentrin specific peptidase 1) from Jinke Cheng (Shanghai Jiao Tong University), and pHA-PA28γ (proteasome activator 28-γ) from Xiaotao Li (East China Normal University). The plasmids expressing the ubiquitin protein ligase E3A (E6AP), heat shock protein 90 (Hsp90), S-phase kinase-associated protein 2 (SKP2), were provided by the Microorganisms & Viruses Culture Collection Center, Wuhan Institute of Virology, CAS.

Free full text: Click here

Guo J., Chen D., Gao X., Hu X., Zhou Y., Wu C., Wang Y., Chen J., Pei R, & Chen X. (2017). Protein Inhibitor of Activated STAT2 Restricts HCV Replication by Modulating Viral Proteins Degradation. Viruses, 9(10), 285.

Publication 2017

Cdna Genotype Heat shock protein 90 Human Human ubiquitin Internal ribosome entry site Peptidase Plasmids Proteasome Proteins Sentrin 1 Skp2 Sumo1 Ubiquitin Ubiquitin protein ligase e3a Viruses

Corresponding Organization : Chinese Academy of Sciences

Other organizations : University of Chinese Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

PHA-PIAS2 (by cloning human PIAS2 cDNA into pXJ40-HA)
Plasmids expressing FLAG-tagged HCV core, E1, E2, NS2, NS3, NS4A, NS4B, NS5A and NS5B proteins of genotype 2a (JFH1)
PNL4.3.lucR-E-
PcDNA3.1-E1E2
PHCV-internal ribosome entry site (IRES)
PUb (by cloning human ubiquitin cDNA into pcDNA3.1)
Human SUMO1 and SUMO2/3 expression plasmids
PSENP1 (SUMO1/sentrin specific peptidase 1)
PHA-PA28γ (proteasome activator 28-γ)
Plasmids expressing ubiquitin protein ligase E3A (E6AP), heat shock protein 90 (Hsp90), S-phase kinase-associated protein 2 (SKP2)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!