miR-485-5p Regulates Cell Proliferation

Twenty-four hours after transfection with miR-485-5p inhibitor, miR-485-5p mimics, miRNA mimic negative controls (NC miR-mimics) and miRNA inhibitor negative controls, NCI-H446 and NCI-H1688 cells were harvested and sub-cultured in 96-well plates. Cell proliferation was assessed using thiazolyl blue tetrazolium bromide assay (MTT; Amresco, Radnor, PA, USA) according to the manufacturer’s instructions. Briefly, MTS reagent (20 µl) was added to each well and incubated at 37°C for 4 h. Then, the reagent was removed and dimethyl sulfoxide (150 µl) was added to each well. Absorbance at 492 nm was measured using the FLx800 fluorescence microplate reader (BioTek, Winooski, VT, USA). The experiment was performed in triplicate and repeated three times. The data are expressed as means ± standard error of the mean (SEM).