Ten-week-old male C57Bl/6J (BL6) mice (purchased from Jackson Laboratory Bar Harbor, ME, USA; Stock No: 000664) were subjected to anterior cruciate ligament injury using a single non-invasive tibial compressive overload, as previously described [12 (link),13 (link),18 (link),19 (link)]. Tibial compression was applied at 1 mm/s, and loading was manually stopped immediately after ACL injury.
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