Drosophila Stock Center Protocol

RNAi lines were ordered from the Vienna Drosophila Resource Center: Patronin IR (VDRC 27654) and Dynein IR (VDRC 28054). The Rab11 RNAi line was generated by Ruth Brain (our laboratory) in the laboratory. UAS.shot-GFP, FRT42B shot³, Ubi.patronin-GFP, UAS.katanin60, GR1.Gal4, FRT19A cdc42³ and FRT40A lgl⁴ were ordered from Bloomington Drosophila Stock Center. UAS.myoV-CT-GFP and UAS.dRip11-CT-GFP lines were gifts from Don Ready (Purdue University, USA) (Li et al., 2007 (link)). The Rab11–YFP line was a gift from Marko Brankatschk, MPI-CBG, Dresden, Germany. Kst–YFP (DGRC 115-285), Tj.Gal4 (DGRC 104-055) and FRT80B nuf (DGRC 111-536) were ordered from the Drosophila Genetic Resource Center, Kyoto. The following strains were used as in previous studies: α-spec^e226 (Hüelsmeier et al., 2007 (link)), kstd1113 (Campos et al., 2010 (link)), kst1 (Thomas and Kiehart, 1994 (link)), Nod.lacZ and Kin.lacZ (Clark et al., 1997 (link)). A list of Drosophila genotypes used in each figure is presented in Table S1.

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Khanal I., Elbediwy A., Diaz de la Loza M.D., Fletcher G.C, & Thompson B.J. (2016). Shot and Patronin polarise microtubules to direct membrane traffic and biogenesis of microvilli in epithelia. Journal of Cell Science, 129(13), 2651-2659.

Publication 2016

GR1.Gal4

Brain Drosophila Dynein Genotypes Gifts Lacz Rnai Strains

Corresponding Organization :

Other organizations : The Francis Crick Institute, The Honourable Society of Lincoln's Inn

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Variable analysis

independent variables

Patronin IR (VDRC 27654)
Dynein IR (VDRC 28054)
Rab11 RNAi line generated by Ruth Brain (our laboratory)
UAS.shot-GFP
Ubi.patronin-GFP
UAS.katanin60
GR1.Gal4
FRT19A cdc42^3
FRT40A lgl^4
UAS.myoV-CT-GFP
UAS.dRip11-CT-GFP
Rab11–YFP
Kst–YFP (DGRC 115-285)
Tj.Gal4 (DGRC 104-055)
FRT80B nuf (DGRC 111-536)
α-spec^e226
Kstd1113
Nod.lacZ
Kin.lacZ

dependent variables

Not explicitly mentioned

control variables

FRT42B shot^3

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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