Microglial and astrocytic activation was assessed using the inflammation markers, Iba1 (1:1000, Wako Ltd., Japan) and GFAP (1:1000, Sigma, Germany) for the fresh-frozen spinal cord slices collected for the autoradiography studies. The immunohistochemistry procedures were performed as described before [22 (link), 23 (link)]. Images of the stained slices were taken with a Panoramic 1000 slide scanner (3DHISTECH).
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