Genetic Labeling of PDGFRα Cells

PDGFRα-Cre^ER mice (Jackson #018280) were crossed to R26-EYFP (Jackson, #006148) animals to generate PDGFRα-Cre^ER; R26-EYFP mice, a previously described model^{21 (link)}. C57B/6J used as adults were purchased from Jackson. Mice were maintained on a 12-h light/dark cycle with lights on at 7am. Behavior was performed on mice used in single-cell sequencing run 1. Testing consisted of sucrose preference, elevated plus maze, open field, and forced swim test. All animal experiments were approved and complied with regulations of the Institutional Animal Care and Use Committee at the University of Virginia (protocol #3918). All experiments were conducted and reported according to ARRIVE guidelines (https://arriveguidelines.org/arrive-guidelines).