Gastrointestinal Protein Localization in Mammals

Localization of the AQP1, AQP3, AQP4, epithelial sodium channel (ENaC) and Na⁺-K⁺-ATPase proteins was evaluated in fixed gastrointestinal tissues of L. yarkandensis and O. cuniculus by immunocytochemistry. Immunocytochemical studies were performed in Paraffin-embedded gastrointestinal tissue, previously fixed in 4% paraformaldehyde. The experimental steps of immunohistochemistry have been described elsewhere^{61 (link)}. The primary antibodies used were as follows: anti-AQP1, anti-AQP3, and anti-AQP4 (diluted to 4.0 μg/ml; Proteintech) and anti-ENaC and anti-Na⁺-K⁺-ATPase (diluted to 3.0 μg/ml; Proteintech). The acquired images were analysed in IpWin32 software^{61 (link)}.

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Zhang J., Li S., Deng F., Baikeli B., Yu W, & Liu G. (2019). Distribution of aquaporins and sodium transporters in the gastrointestinal tract of a desert hare, Lepus yarkandensis. Scientific Reports, 9, 16639.

Publication 2019

anti-AQP1 anti-Na+-K+-ATPase

Antibodies Aqp1 Cuniculus Embedded paraffin Epithelial sodium channel Immunocytochemistry Immunohistochemistry Na k atpase Paraformaldehyde Proteins Tissue

Corresponding Organization : Tarim University

Other organizations : Bengbu Medical College

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Variable analysis

independent variables

Tissue type (L. yarkandensis and O. cuniculus gastrointestinal tissues)

dependent variables

Localization of AQP1, AQP3, AQP4, epithelial sodium channel (ENaC), and Na+-K+-ATPase proteins in the gastrointestinal tissues

control variables

Fixation of gastrointestinal tissues in 4% paraformaldehyde
Paraffin-embedding of gastrointestinal tissues
Dilution of primary antibodies (anti-AQP1, anti-AQP3, anti-AQP4, anti-ENaC, and anti-Na+-K+-ATPase)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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