Formalin-fixed paraffin-embedded tissue sections of 5 μm thickness from the primary motor cortex of three HD autopsy cases and three age-matched controls were provided by the Neurobiobank Munich, Ludwig-Maximilians-Universität München. Informed consent was available for all cases. The experiments were approved by the ethics committee of the Max Planck Society and performed in accordance with the relevant guidelines and regulations. All HD cases were symptomatic; demographic information is described in Burgold et al (2019) (link).
Immunohistochemistry was performed on a VENTATA BenchMark ULTRA (Roche). After standard pretreatment in CC1 buffer (Roche), sections were incubated with rabbit anti-HDGF antibody (ab244498; Abcam) at a dilution of 1:100 for 32 min. The UltraView Universal DAB Detection Kit (Roche) was used for detection and counterstaining was performed with hematoxylin for 4 min. Images were acquired with a Leica THUNDER imager. Control stainings without the primary antibody were imaged using an Olympus BX45 microscope with Olympus DP32 camera.
Immunohistochemistry was performed on a VENTATA BenchMark ULTRA (Roche). After standard pretreatment in CC1 buffer (Roche), sections were incubated with rabbit anti-HDGF antibody (ab244498; Abcam) at a dilution of 1:100 for 32 min. The UltraView Universal DAB Detection Kit (Roche) was used for detection and counterstaining was performed with hematoxylin for 4 min. Images were acquired with a Leica THUNDER imager. Control stainings without the primary antibody were imaged using an Olympus BX45 microscope with Olympus DP32 camera.
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