Comprehensive Metabolomics Analysis Protocol

Metabolomics analysis was performed using a previously established method^{22 (link)}. Briefly, the metabolomics analysis, ultra-high performance liquid chromatography (Shimadzu Nexera system; Shimadzu; Columbia, MD) coupled to a high resolution hybrid quadrupole-time-of-fight (TOF) mass spectrometer (MS) (TripleTOF 5600; Sciex; Framingham, MA) was utilized. Every sample underwent analysis via two different methods using either reverse phase and HILIC columns in both the negative and positive ion modes^{21 (link)}. High performance liquid chromatography (Agilent 1100 Series) coupled to LTQ-XL (Thermo Fisher) was used to analyze plasma levels of phosphatidylcholine and lysophosphatidylcholine^{67 (link)}. Peak area of each metabolite, measured within the linear range of the mass detector, was normalized to plasma volume for each sample and presented as mean ± SEM.

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Shoaib M., Choudhary R.C., Choi J., Kim N., Hayashida K., Yagi T., Yin T., Nishikimi M., Stevens J.F., Becker L.B, & Kim J. (2020). Plasma metabolomics supports the use of long-duration cardiac arrest rodent model to study human disease by demonstrating similar metabolic alterations. Scientific Reports, 10, 19707.

Publication 2020

Nexera system TripleTOF 5600 Agilent 1100 Series LTQ-XL

High performance liquid chromatography Hybrid Phosphatidylcholine Plasma Plasma volume

Corresponding Organization :

Other organizations : Feinstein Institute for Medical Research, Oregon State University, Donald & Barbara Zucker School of Medicine at Hofstra/Northwell

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Metabolite peak area
Plasma levels of phosphatidylcholine and lysophosphatidylcholine

control variables

Plasma volume

controls

None explicitly mentioned

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