Glutamate-Induced Cell Viability Assay

Cell viability in HT-22 cells was investigated according to a previously reported method [10 (link)]. Briefly, the HT-22 cells were seeded into 96-well plates at a density of 3 × 10³ cells/well for 24 h, with each well containing 100 μL of medium. The cells were treated with glutamate (5 mM) in the presence or absence of RDP (5–50 μg/mL). After 11 h of incubation, cell viability was evaluated using the EZ-Cytox Cell Viability Assay Kit following the manufacturer’s instructions. The absorbance was measured by an Emax Precision microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm. The percentage of surviving cells was calculated relative to the values of vehicle control group.