Construction of Inducible Fluorescent Reporters

Protein expression vector pMAL-c5Xa (NEB) was used as the backbone for constructing the mCherry reporter. The malE gene and the lacI promoter were restricted from the vector using SacI and KasI restriction enzymes. The entCEBA promoter was amplified with primers GK073-F/GK073-R (Table S1) and inserted into the pMAL-c5Xa backbone. The reporter constructs were transformed into respective strains as indicated. An inducible GFP was made by inserting the Yersinia operon 1 promoter sequence into plasmid pFCcGi (Addgene) upon restriction with HindIII and XbaI (NEB). The resulting construct was called pybtP:GFP (30 (link)). The reporter constructs were transformed into respective strains as indicated.