The measurement of antiradical activity of samples before and after digestion was carried out via DPPH stable radical (2,2-diphenyl-1- picrylhydrazyl) spectroscopy [3 (link)].
Absorbance was measured at a wavelength of 517 nm, and the UV-VIS spectrophotometer (Genesys 150 UV-VIS, Thermo Scientific, Waltham, MA, USA) was calibrated to pure methanol. Measurements were performed every 5 min until a plateau was reached. This allowed the changes in absorbance to be monitored over time and indicated when the plateau was reached. The following formula was used to calculate the radical scavenging potential of the extracts:
Absorbance was measured at a wavelength of 517 nm, and the UV-VIS spectrophotometer (Genesys 150 UV-VIS, Thermo Scientific, Waltham, MA, USA) was calibrated to pure methanol. Measurements were performed every 5 min until a plateau was reached. This allowed the changes in absorbance to be monitored over time and indicated when the plateau was reached. The following formula was used to calculate the radical scavenging potential of the extracts:
A0—the absorbance of the sample except for the tested extracts;
A1—the absorbance of the sample with tested extracts.
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