Second Harmonic Generation Imaging of H&E Sections

Second harmonic generation (SHG) images of H&E sections were taken using a Zeiss Axio Examiner Z1 microscope with 20× water immersion objective (1.0 NA DIC [UV] VIS-IR) as described (33 (link)). The SHG signal was obtained using a band pass cube of 380–430nm. Images were taken with a Coherent Chameleon Verdi laser at 820 nm wavelength. Z-stack images were taken with a 1μm interval.

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Richardson A.M., Havel L.S., Koyen A.E., Konen J.M., Shupe J., Wiles WG I.V., Martin W.D., Grossniklaus H.E., Sica G., Gilbert-Ross M, & Marcus A.I. (2017). Vimentin is required for lung adenocarcinoma metastasis via heterotypic tumor cell-cancer-associated fibroblast interactions during collective invasion. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(2), 420-432.

Publication 2017

Axio Examiner Z1 microscope

Chameleon Immersion Microscope

Corresponding Organization :

Other organizations : Emory University

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Variable analysis

independent variables

Wavelength of the Coherent Chameleon Verdi laser (820 nm)

dependent variables

Second harmonic generation (SHG) signal
SHG images of H&E sections

control variables

Zeiss Axio Examiner Z1 microscope with 20× water immersion objective (1.0 NA DIC [UV] VIS-IR)
Band pass cube of 380–430nm for SHG signal detection
Z-stack images taken with 1μm interval

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