Oxygen consumption rate (OCR) was assessed using the Seahorse XF96 (Seahorse Bioscience), following the protocol outlined in (Koopman et al., 2016 (link)). Briefly, a synchronized culture of ~100 worms was harvested on day 1 of adulthood with sterile M9 buffer. After three washes in the M9 buffer, the worms were transferred to a 96-well Seahorse plate, where their OCR was measured six times to determine mitochondrial activity for each condition at basal level and another six times measurement after adding 10 μM FCCP as the final concentration.
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