Live-cell Imaging for Cell Proliferation and Migration

Proliferation rates, which were based on cell confluence, were determined by live-cell imaging (IncuCyte ZOOM System, Essen BioScience, Ann Arbor, MI, USA), as described previously [32 (link)]. To analyze cell migration, the cells were cultured in 96-well ImageLock Plates (Essen BioScience) to reach confluence prior to wound creation. A scratch was made in confluent monolayers while using a 96-pin WoundMaker (Essen BioScience, Ann Arbor, MI, USA). The cells were washed with PBS and then incubated using the IncuCyte ZOOM System. Cell migration was analyzed at 2 h intervals throughout the duration of the experiment.

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Kim B.K., Cheong J.H., Im J.Y., Ban H.S., Kim S.K., Kang M.J., Lee J., Kim S.Y., Park K.C., Paik S, & Won M. (2019). PI3K/AKT/β-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer. Cancers, 11(12), 1923.

Publication 2019

IncuCyte ZOOM System ImageLock Plates 96-pin WoundMaker

Cell migration Cells Wound

Corresponding Organization : Korea University of Science and Technology

Other organizations : Yonsei University

Top 5 similar protocols

Variable analysis

independent variables

Proliferation rates
Cell migration

dependent variables

Cell confluence
Cell migration

control variables

Culturing cells in 96-well ImageLock Plates
Creating a scratch in confluent monolayers using a 96-pin WoundMaker
Incubating cells using the IncuCyte ZOOM system

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